ISSN 2149-3235 | E-ISSN 2149-3057
Original Article
Protective role of resveratrol on testicular germ cells in mice with testicular toxicity
1 Department of Pharmacology, Ahi Evran University School of Medicine, Kırsehir, Turkey  
2 Department of Histology and Embriyology, Ahi Evran University School of Medicine, Kırsehir, Turkey  
3 Department of Pharmacology, Eskişehir Osmangazi University School of Medicine, Eskişehir, Turkey  
Turk J Urol 2017; 43: 444-450
DOI: 10.5152/tud.2017.34101
Key Words: Mice; resveratrol; testis; toxicity

Objective: The aim of the present study was to investigate the possible beneficial effects of resveratrol in mice subjected to vinyl cyclohexene dieposide (VCD) -induced testicular toxicity.


Material and methods: A total of thirty- six Swiss albino male mice aged 28-days were used in the present study. The study was composed of two stages where mice which received or did not receive VCD (320 mg/kg/day) were administered resveratrol. The animals were assigned into control and resveratrol-treated groups in the first stage and into groups of VCD- and VCD+resveratrol-treated groups in the second stage. At the end of the experiments, relative testicular weight (TW/BW) and dry/wet weight of testis (TDW/TWW) were calculated. Histological analysis by hematoxylin and eosin (H&E) staining and immunohistochemical staining by BAX and Bcl-2 were performed. Serum testosterone, LH and FSH levels were measured by a commercially available ELISA kit.


Results: Resveratrol caused a dose-dependent increase in TW/BW and decrease in TDW/TWW (p<0.05). Resveratrol at a dose of 20 mg/kg resulted in an improvement in testosterone, LH and FSH levels in mice with VCD-induced testicular toxicity (p<0.001). Resveratrol also improved apoptotic index and epithelial cell height of testicular seminipherous tubuli significantly after VCD exposure (p<0.001).



Conclusion: Results of the present study suggest that resveratrol can be used as a protective and/or therapeutic agent particularly for cases with male infertility caused by testicular toxicity.



Cite this article as: Özatik FY, Özatik O, Yiğitaslan S, Çengelli Ünel Ç, Erol K. Protective role of resveratrol on testicular germ cells in mice with testicular toxicity. Turk J Urol 2017; 43(4): 444-50.

Key Words
AVES | Copyright © 2018 Turkish Association of Urology | Latest Update: 08.10.2018