ISSN 2149-3235 | E-ISSN 2149-3057
Original Article
Detection of CYP1A1 and GSTP1 gene polymorphisms in bladder cancer patients in a Turkish population using a polymerase chain reaction-restriction fragment length polymorphism method
1 Department of Urology, University of Health Sciences, Adana Numune Training and Research Hospital, Adana, Turkey  
2 Department of Urology, University of Health Sciences, Antalya Training and Research Hospital, Antalya, Turkey  
3 Department of Medical Biology and Genetics, Harran University School of Medicine, Şanlıurfa, Turkey  
4 Department of Childhood Urology, Uludağ University School of Medicine, Bursa, Turkey  
5 Clinic of Urology, Ministry of Health, Balıklıgöl State Hospital, Şanlıurfa, Turkey  
6 Department of Urology, University of Health Sciences, Mehmet Akif İnan Training and Research Hospital, Şanlıurfa, Turkey  
7 Department of Urology, Harran University Faculty of Medicine, Şanlıurfa, Turkey  
Turk J Urol 2018; 44: 125-131
DOI: 10.5152/tud.2018.23571
Key Words: Bladder cancer; cytochrome; gene; glutathione; polymorphism
Abstract

 

Objective: Understanding genetic polymorphisms might facilitate the analysis of differences between individuals in their susceptibility to developing cancers as a result of environmental carcinogens. Skin, lung, colon and bladder cancers emerge from biological defects in GSTM1, GSTT1 and GSTP1 gene expressions. In this study, we aimed to investigate whether there was an association between CYP1A1 and GSTP1 gene polymorphisms and bladder cancer in a Turkish population.

 

Material and methods: Blood samples were collected from 120 individuals (60 patients with bladder cancer and 60 healthy individuals), and their DNAs were isolated. A polymerase chain reaction-restriction fragment length polymorphism (PCR - RFLP) method was used to detect the frequencies of CYP1A1 NM_000499.3: c.*1189T > C and GSTP1 NM_000852.3: c.313A > G polymorphisms in bladder cancer patients.

 

Results: The frequency of the CYP1A1: c.*1189 TC genotype and C allele were significantly different between bladder cancer patients and healthy individuals (p=0.001 and p=0.005, respectively). However, there was no significant difference for the GSTP1: c.313 AG genotype or G allele between both study groups (p=0.699 and p=0.360, respectively).

 

Conclusion: A polymorphic site of the CYP1A1 gene might be involved in the development of bladder cancer. However, the investigated GSTP1 polymorphic site did not represent an important risk factor for the development of bladder cancer in a Turkish population.

 

Cite this article as: Altunkol A, Savaş M, Dilmeç F, Utanğaç MM, Abat D, Gümüş K, et al. Detection of CYP1A1 and GSTP1 gene polymorphisms in bladder cancer patients in a Turkish population using a polymerase chain reaction-restriction fragment length polymorphism method. Turk J Urol 2018; 44(2): 125-31.

Key Words
Authors
All
Survey
AVES | Copyright © 2018 Turkish Association of Urology | Latest Update: 08.10.2018