ISSN 2149-3235 | E-ISSN 2149-3057
Original Article
Is there a difference in number of interstitial cells, neurons, presence of fibrosis and inflammation in UPJ tissues of patients with UPJ obstruction with and without crossing-vessel and normal subjects in humans?
1 Department Of Pathology, Ankara Yıldırım Beyazıt University, Ankara, Turkey  
2 Department Of Urology, Ankara Yıldırım Beyazıt University, Ankara, Turkey  
3 Department of Urology, Ankara Atatürk Training and Research Hospital, Department of Urology, Ankara, Turkey  
4 Department of Urology, Giresun University, Giresun, Turkey  
5 Department of Pathology, Cukurova Dr. Askim Tufekci State Hospital, Adana, Turkey  
6 Department of Pathology, Sakarya Training and Research Hospital, Sakarya, Turkey  
Turk J Urol ; : -
DOI: 10.5152/tud.2018.08784
Key Words: interstitial cells, neurons, fibrosis, inflammation, UPJ obstruction
Abstract

 

Objective: We compared the number of interstitial cells (ICs), nerves, presence of fibrosis and inflammation at the level of full thickness human uretero-pelvic junction (UPJ) tissues obtained from normal subjects, UPJ obstruction with crossing-vessel and UPJ obstruction without crossing-vessel.

 

Methods: Normal UPJ tissues (n=12) without tumor involvement confirmed histopathologically were obtained from subjects who underwent radical nephrectomy for kidney mass. Additional UPJ tissues were obtained from patients who underwent pyeloplasty due to UPJ obstruction. Crossing vessel was identified in 17 patients. In 57 patients, no crossing-vessel was noted. The mean age of total population included in the study was 30,5±18,5. ICs were stained immunohistochemically with anti–human CD117 (c–kit) antibody. Neural tissue was stained with S-100. The numbers of ICs and neurons
were compared between the groups: controls with normal UPJ (Group I), UPJ obstruction with crossing vessel (Group II) and UPJ obstruction without crossing vessel (Group III). Groups were also compared in terms of the presence of fibrosis and inflammation.

 

Results: No significant differences were detected between the three groups regarding mean and median numbers of ICs at the level of UPJ (lamina propria & muscle layer) and mean and median numbers of neurons at the level of lamina propria (p>0.05). Likewise, no significant differences were detected between the three groups regarding the presence of fibrosis and inflammation (p>0.05).

 

Conclusions: Number of ICs, neurons, presence of fibrosis and inflammation are seem to be similar in the healthy UPJ and UPJ with obstruction with and without crossing vessel. Cellular function rather than number might play a role that warrants further research.

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